研究業績

2019年

  • Tanaka-Takada N., Kobayashi A., Takahashi H., Kamiya T., Kinoshita T., Maeshima M.   Plasma membrane-associated Ca2+-binding protein PCaP1 is involved in root hydrotropism of Arabidopsis thaliana.  Plant and Cell Physiology   doi:10.1093/pcp/pcz042.

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    2018年

  • Kobayashi A., Kim H-J., Tomita Y., Miyazawa Y., Fujii N., Yano S., Yamazaki C., Kamada M., Kasahara H., Miyabayashi S., Shimazu T., Fusejima Y., Takahashi H.   Circumnutational movement in rice coleoptiles involves the gravitropic response: analysis of an agravitropic mutant and space-grown seedlings.  Physiologia Plantarum   doi: 10.1111/ppl.12824.
  • Kojima S., Iwamoto M., Oiki S., Tochigi S., Takahashi H.   Thylakoid membranes contain a non-selective channel permeable to small organic molecules.  Journal of Biological Chemistry 293(20):7777-7785.   doi: 10.1074/jbc.RA118.002367
  • Fujii N., Miyabayashi S., Sugita T., Kobayashi A., Yamazaki C., Miyazawa Y., Kamada M., Kasahara H., Osada I., Shimazu T., Fusejima Y., Higashibata A., Yamazaki T., Ishioka N., Takahashi H.   Root-tip-mediated inhibition of hydrotropism is accompanied with the suppression of asymmetric expression of auxin-inducible genes in response to moisture gradients in cucumber roots.  PLoS One 13(1):e0189827   DOI: 10.1371/journal.pone.0189827. eCollection 2018.

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    2017年

  • Kowata H., Tochigi S.,Takahashi H. and Kojima S.   Outer membrane permeability of cyanobacterium Synechocystis sp. PCC 6803: studies of passive diffusion of small organic nutrients reveal the absence of classical porins and intrinsically low permeability.  Journal of Bacteriology   DOI: 10.1128/JB.00371-17
  • Morohashi K., Okamoto M., Yamazaki C., Fujii N., Miyazawa Y., Kamada M., Kasahara H., Osada I., Shimazu T., Fusejima Y., Higashibata A., Yamazaki T., Ishioka N., Kobayashi A., and Takahashi H.   Gravitropism interferes with hydrotropism via counteracting auxin dynamics in cucumber roots: Clinorotation and spaceflight experiments.  New Phytologist   215(4):1476-1489.   doi: 10.1111/nph.14689
  • Kohzuma K., Chiba M., Nagano S., Anai T., Ueda M.U., Oguchi R., Shirai K., Hanada K., Hikosaka K., Fujii N.   Mutant selection in the self-incompatible plant radish (Raphanus sativus L. var. sativus) using two-step TILLING.  Breeding Science   67(3):268-276.   doi: http://doi.org/10.1270/jsbbs.16200
  • Nakajima Y.*, Nara Y.*, Kobayashi A.*, Sugita T., Miyazawa Y., Fujii N. and Takahashi H.   Auxin transport and response requirements for root hydrotropism differ between plant species.  Jornal of Experimental Botany   68(13):3441-3456. doi: 10.1093/jxb/erx193.  *equal contribution
  • Dietrich D.*, Pang L.*, Kobayashi A.*, Fozard J.A., Boudolf V., Bhosale R., Antoni R., Nguyen T., Hiratsuka S., Fujii N., Miyazawa Y., Bae T-W., Wells D.M., Owen M.R., Band L.R., Dyson R.J., Jensen O.E., King J.R., Tracy S.R., Sturrock C.J., Mooney S.J., Roberts J.A., Bhalerao R.P., Dinneny J.R., Rodriguez P.L., Nagatani A., Hosokawa Y., Baskin T.I., Pridmore T.P., Veylder L.D., Takahashi H. and Bennett M.J.   Root hydrotropism is controlled via a cortex-specific growth mechanism.  Nature Plants  3: 17057 DOI: 10.1038/nplants.2017.57  *equal contribution

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    2016年

  • Sugawara, E., Kojima, S., Nikaido, H.   Klebsiella pneumoniae major porins OmpK35 and OmpK36 allow more efficient diffusion of β-lactams than their Escherichia coli homologs OmpF and OmpC.  Journal of Bacteriology  198(23):3200-3208
  • Yamazaki, C., Fujii, N., Miyazawa, Y., Kamada, M., Kasahara, H., Osada, I., Shimazu, T., Fusejima, Y., Higashibata, A., Yamazaki, T., Ishioka, N., Takahashi, H.   The gravity-induced re-localization of auxin efflux carrier CsPIN1 in cucumber seedlings: spaceflight experiments for immunohistochemical microscopy.   npj Microgravity 2  Article number: 16030
  • Kojima, S., Muramoto, K., Kusano,T.   Outer Membrane Proteins Derived from Non-cyanobacterial Lineage Cover the Peptidoglycan of Cyanophora paradoxa Cyanelles and Serve as a Cyanelle Diffusion Channel.  The journal of Biological Chemistry   291(38):20198-20209.
  • Kojima, S., Hayashi, K., Tochigi, S., Kusano, T., Kaneko, J., Kamio, Y.  Peptidoglycan-associated outer membrane protein Mep45 of rumen anaerobe Selenomonas ruminantium forms a non-specific diffusion pore via its C-terminal transmembrane domain.  Bioscience, Biotechnology, and Biochemistry  80:1954-1959.
  • Kowata, H., Tochigi, S., Kusano, T., Kojima, S.  Quantitative measurement of the outer membrane permeability in Escherichia coli lpp and tol-pal mutants defines the significance of Tol-Pal function for maintaining drug resistance.  The journal of Antibiotics   Advance online publication 11 May 2016
  • G.H.M., Sagor., Berberich, T., Kojima, S., Niitsu, M., Kusano, T.  Spermine modulates the expression of two probable polyamine transporter genes and determines growth responses to cadaverine in Arabidopsis.   Plant Cell Reports   35:1247-1257.
  • G.H.M., Sagor., Zhang, S., Kojima, S., Simm, S., Berberich, T., Kusano, T. Reducing cytoplasmic polyamine oxidase activity in Arabidopsis increases salt and drought tolerance by reducing reactive oxygen species production and increasing defense gene expression.   Frontiers in Plant Science   7:214.
  • G.H.M., Sagor., Berberich, T., Tanaka, S., Nishiyama, M., Kanayama, Y., Kojima, S., Muramoto, K., Kusano, T.  A novel strategy to produce sweeter tomato fruits with high sugar contents by fruit-specific expression of a single bZIP transcription factor gene.  Plant Biotechnology Journal   14:1116-1126.
  • 児島征司  グラム陰性細菌の多剤耐性― β-lactam 系抗生物質の外膜透過・排出速度の測定結果を例に理解する―  化学と生物  54(2):80-82.
  • Kim, H.J. Kobayashi, A., Fujii, N., Miyazawa, Y., Takahashi, H.  Gravitropic response and circumnutation in pea (Pisum sativum) seedling roots.   Physiologia Plantarum  157: 108–118.

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    2014年

  • Moriwaki, T., Miyazawa, Y., Fujii, N., Takahashi, H.  GNOM regulates root hydrotropism and phototropism independently of PIN-mediated auxin transport.   Plant Science  215-216: 141-149.

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    2013年

  • Yamazaki, C., Fujii N., Takahashi, H.  Polar Auxin Transport: The Role of Auxin Transport and Distribution in Plant Gravimorphogenesis."   Springer Berlin Heidelberg  25-44.
  • Iwata, S., Miyazawa, Y., Fujii N., Takahashi, H.  MIZ1-regulated hydrotropism functions in the growth and survival of Arabidopsis thaliana under natural conditions.  Annals of Botany 112 (1): 103-114
  • Moriwaki, T., Miyazawa, Y., Kobayashi, A., Takahashi, H.  Molecular mechanisms of hydrotropism in seedling roots of Arabidopsis thaliana (Brassicaceae). American Journal of Botany 100.1: 25-34

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    2012年

  • Miyazawa, Y., Moriwaki, T., Uchida, M., Kobayashi, A., Fujii N., Takahashi, H.  Overexprresion of MIZU-KUSSEI1 enhances root hydrotropic response by retaining cell viability under hydrostimulated condition in Arabidopsis thaliana. Plant and Cell Physiology 53(11): 1926-1933
  • Nakayama, M., Kaneko, Y., Miyazawa, Y., Fujii, N., Higashitani, N., Wada, S., Ishida, H., Yoshimoto, K., Shirasu, K., Yamada, K., Nishimura, M., Takahashi, H.  A possible involvement of autophagy in amyloplast degradation in columella cells during hydrotropic response of Arabidopsis roots. Planta 236: 999-1012
  • Moriwaki, T., Miyazawa, Y., Fujii N., Takahashi, H.  Light and abscisic acid signaling are integrated by MIZ1 gene expression and regulate hydrotropic response in roots of Arabidopsis thaliana.  Plant Cell and Environment 20: 79-83.
  • Yamazaki, T., Miyazawa, Y., Kobayashi, A., Moriwaki, T., Fujii, N., Takahashi, H.  MIZ1, an essential protein for root hydrotropism, is associated with the cytoplasmic face of the endoplasmic reticulum membrane in Arabidopsis thaliana.  FEBS Letters  586: 398-402
  • Watanabe, C., Fujii, N., Yanai, K., Hotta, T., Kim, D-H., Kamada, M., Sasagawa-Saito, Y., Nishimura, T., Koshiba, T., Miyazawa, Y., Kim, K-M., Takahashi, H.  Gravistimulation changes the accumulation pattern of the CsPIN1 auxin efflux facilitator in the endodermis of the transition zone in cucumber seedlings.  Plant Physiology 158: 239-251
  • Iwata, S., Miyazawa, Y., Takahashi, H. MIZU-KUSSEI1 plays an essential role in the hydrotropism of lateral roots in Arabidopsis thaliana.  Environmental and Experimental Botany 75: 167-172
  • 山崎聖司、真鍋和人、藤井伸治、高橋秀幸. ウリ科植物の花の性分化のエチレン制御と遺伝モデル.  植物の生長調節 47(1) : 24-33

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    2011年

  • Moriwaki, T., Miyazawa, Y., Kobayashi, A., Uchida, M., Watanabe, C., Fujii, N., Takahashi, T.  Hormonal regulation of lateral root development in Arabidopsis modulated by MIZ1 and requirement of GNOM activity for MIZ1 function.  Plant Physiology 157: 1209-1220
  • Miyazawa, Y., Yamazaki, T., Moriwaki, T., Takahashi, H. Root tropisms: its mechanism and possible functions in drought avoidance. In Turkan I. (eds.) Plant responses to drought and salinity stress: development in a post-genomic era, Advances in Botanical Research 57: 349-375
  • Kato, F., Araki, M., Miyazawa, Y., Fujii, N., Takeda, K., Suge, H., Takahashi, H. Factors responsible for deep-sowing tolerance in wheat seedlings: Varietal differences in cell proliferation and the coordinated synchronization of epidermal cell expansion and cortical cell division for the gibberellin-mediated elongation of first internodes. Annals of Botany 108: 439-447

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    2010年

  • Sasagawa-Saito, Y., Kitazawa, D., Miyazawa, Y., Fujii, N., Takahashi, H. Differential accumulation of CsARF5 protein during peg formation in cucumber seedlings. Biological Sciences in Space 24: 153-162
  • Sakata, T., Oshino, T., Miura, S., Tomabechi, M., Tsunaga, Y., Higashitani, N., Miyazawa, Y., Takahashi, H., Watanabe, M., Higashitani, A. Auxins reverse plant male sterility caused by high temperatures. Proceedings of the National Academy of Sciences of the U.S.A. 107: 8569-8574
  • Moriwaki, T., Miyazawa, Y., Takahashi, H. Transcriptome analysis of gene expression during the hydrotropic response in Arabidopsis seedlings. Environmental and Experimental Botany 69: 148-157
  • Nishio, S., Moriguchi, R., Ikeda, H., Takahashi, H., Takahashi, H., Fujii, N., Guilfoyle, T.J., Kanahama, K., Kanayama, Y. Expression analysis of the auxin efflux carrier family in tomato fruit development. Planta 232: 755-764

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    2009年

  • Miyazawa, Y., Takahashi, A., Kobayashi, A., Kaneyasu, T., Fujii, N., Takahashi, H. GNOM-mediated vesicular trafficking plays an essential role in hydrotropism of Arabidopsis roots. Plant Physiology 149: 835-840
  • Miyazawa, Y., Ito, Y., Moriwaki, T., Kobayashi, A., Fujii, N., Takahashi, H. A molecular mechanism unique to hydrotropism in roots. Plant Science 177: 297-301
  • Takahashi, H., Miyazawa, Y., Fujii, N. Hormonal interactions during root tropic growth: hydrotropism versus gravitropism. Plant Molecular Biology 69: 489-502

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    2008年

  • Miyazawa, Y., Sakashita, T., Funayama, T., Hamada, N., Negishi, H., Kobayashi, A., Kaneyasu, T., Ooba, A., Morohashi, K., Kakizaki, T., Wada, S., Kobayashi, Y., Fujii, N., Takahashi, H. Effects of locally targeted heavy-ion and laser microbeam on root hydrotropism in Arabidopsis thaliana. Journal of Radiation Research 49: 373-379
  • Kitazawa, D., Miyazawa, Y., Fujii, N., Hoshino, A., Iida, S., Nitasaka, E., Takahashi, H. The gravity-regulated growth of axillary bud is mediated by a mechanism different from decapitation-induced release. Plant and Cell Physiology 49: 891-900
  • Shimizu, M., Miyazawa, Y., Fujii, N., Takahashi, H. p-Chlorophenoxy- isobutyric acid impairs auxin response for gravity-regulated peg formation in cucumber seedlings. Journal of Plant Research 121: 107-114
  • Kitazawa, D., Miyazawa, Y., Fujii, N., Nitasaka, E., Takahashi, H. Characterization of a novel gravitropic mutant of morning glory, weeping2. Advances in Space Research 42: 1050-1059

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    2007年

  • Kobayashi, A., Takahashi, A., Kakimoto, Y., Miyazawa, Y., Fujii, N., Higashitani, A., Takahashi, H. A gene essential for hydrotropism in roots. Proceedings of the National Academy of Sciences of the U.S.A. 104: 4724-4729
  • Saito, S., Fujii, N., Miyazawa, Y., Yamasaki, S., Matsuura, S., Mizusawa, H., Fujita, Y., Takahashi, H. Correlation between development of female flower buds and expression of the CS-ACS2 gene in cucumber plants. Journal of Experimental Botany 58: 2897-2907
  • Kaneyasu, T., Kobayashi, A., Nakayama, M., Fujii, N., Takahashi, H., Miyazawa, Y. Auxin response, but not its polar transport, plays a role in hydrotropim of Arabidopsis thaliana. Journal of Experimental Botany 58: 1143-1150
  • Miyazawa, Y., Takahashi, H. How do Arabidopsis roots differentiate hydrotropism from gravitropism? Plant Signaling and Behavior 2: 388-389
  • Fujii, N., Saito, Y., Miyazawa, Y., Takahashi, H. Light inhibits gravity-regulated peg formation and asymmetric mRNA accumulation of auxin-inducible CsIAA1 in the cortex of the transition zone in cucumber seedlings. Advances in Space Research 39: 1147-1153
  • Shimizu, M., Suzuki, K., Miyazawa, Y., Fujii, N., Takahashi, H. Differential accumulation of the mRNA of the auxin-repressed gene CsGRP1 and the auxin-induced peg formation during gravimorphogenesis of cucumber seedlings. Planta 225: 13-22

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    2006年

  • Kitaya, Y., Kawai, M., Takahashi, H., Tani, A., Goto, E., Saito, T., Shibuya, T., Kiyota, M. Heat and gas exchanges between plants and atmosphere under microgravity. Annals of New York Academy of Sciences 1077: 244-255
  • Masuko, H., Endo, M., Saito, H., Hakozaki, H., Park, J-I., Kawagishi-Kobayashi, M., Takada, Y., Okabe, T., Kamada, M., Takahashi, H., Higashitani, A., Watanabe, M. Anther-specific genes, which expressed through microsporogenesis, are temporally and spatially regulated in model legume, Lotus japonicus. Genes and Genetic Systems 81: 57-62

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    2005年

  • Kitazawa, D., Hatakeda, Y., Kamada, M., Fujii, N., Miyazawa, Y., Hoshino, A., Iida, S., Fukaki, H., Morita, M.T., Tasaka, M., Suge, H., Takahashi, H. Shoot circumnutation and winding movements require gravisensing cells. Proceedings of the National Academy of Sciences of the U.S.A.102: 18742-18747
  • Abiko, M., Akibayashi, K., Sakata, T., Kimura, M., Kihata, M., Itoh, K., Asamizu, E., Sato, S., Takahashi, H., Higashitani, A. High-temperature induction of male sterility during barley (Hordeum vulgare L.) anther development is mediated by transcriptional inhibition. Sexual Plant Reproduction 18: 91-100
  • Sasagawa, Y., Saito, Y., Shimizu, M., Takahashi, H., Higashitani, A. Perturbation of oocyte meiotic division by hypergravity in Caenorhabditis elegans. Biological Sciences in Space 19: 155-162
  • 高橋秀幸、藤井伸治、宮沢豊 「微小重力下における根の水分屈性とオーキシン動態」 生物工学 83: 560-564
  • Saito, Y., Yamasaki, S., Fujii, N., Takahashi, H. Possible involvement of CS-ACS1 and ethylene in auxin-induced peg formation of cucumber seedlings. Annals of Botany 95: 413-422
  • Yamasaki, S., Fujii, N., Takahashi, H. Hormonal regulation of sex expression in plants. Vitamins and Hormones vol. 72: 79-110

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    2004年

  • Saito, Y., Yamasaki, S., Fujii, N., Hagen, G., Guilfoyle, T., Takahashi, H. Isolation of cucumber CsARF cDNAs and expression of the corresponding mRNAs during gravity-regulated morphogenesis of cucumber seedlings. Journal of Experimental Botany 55: 1315-1323
  • Hakozaki, H., Endo, M., Masuko, H., Park, J-I., Ito, H., Uchida, M., Kamada, M., Takahashi, H., Higashitani, A., Watanabe, M. Cloning and expression pattern of a novel microspore-specific gene encoding hypersensitive-induced response protein (LjHIR1) from the model legume, Lotus japonicus. Genes and Genetic Systems 79: 307-310
  • Endo, M., Tsuchiya, T., Saito, H., Matsubara, H., Hakozaki, H., Masuko, H., Kamada, M., Higashitani, A., Takahashi, H., Fukuda, H., Demura, T., Watanabe, M. Identification and molecular characterization of novel anther-specific genes in Oryza sativa L. by using cDNA microarray. Genes and Genetic Systems 79: 213-226

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    2003年

  • Kamada, M., Yamasaki, S., Fujii, N., Higashitani, A., Takahashi, H. Gravity-induced modification of auxin transport and distribution for peg formation in cucumber seedlings: possible roles for CS-AUX1 and CS-PIN1. Planta 218: 15-26
  • Takahashi, N., Yamazaki, Y., Kobayashi, A., Higashitani, A., Takahashi, H. Hydrotropism interacts with gravitropism by degrading amyloplasts in seedling roots of Arabidopsis and radish. Plant Physiology 132: 805-810
  • Yamasaki, S., Fujii, N., Takahashi, H.  Photoperiodic regulation of CS-ACS2, CS-ACS4, and CS-ERS gene expression contributions to the femaleness of cucumber flowers through diurnal ethylene production under short-day conditions.  Plant Cell and Environment 26: 537-546.
  • Yamasaki, S., Fujii, N., Takahashi, H. Characterization of ethylene effects on sex determination in cucumber plants. Sexual Plant Reproduction 16: 103-111.
  • Hatakeda, Y., Kamada, M., Goto, N., Fukaki, H., Tasaka, M., Suge, H. Takahashi, H. Gravitropic response plays an important role in the nutational movements of the shoots of Pharbitis nil and Arabidopsis thaliana. Physiologia Plantarum 118: 464-473
  • Chen, L., Higashitani, A., Suge, H., Takeda, K., Takahashi, H. Spiral growth and cell wall properties of the gibberellin-treated first internodes in the seedlings of a wheat cultivar tolerant to deep-sowing conditions. Physiologia Plantarum 118: 147-155
  • Tsuda, S., Miyamoto, N., Takahashi, H., Ishihara, K., Hirasawa, T. Roots of Pisum sativum L. exhibit hydrotropism in response to a water potential gradient in vermiculite. Annals of Botany 92: 767-770
  • Takanami, T., Zhang, Y., Aoki, H., Abe, T., Yoshida, S., Takahashi, H., Horiuchi, S., Higashitani, A. Efficient repair of DNA damage induced by heavy ion particles in meiotic prophase I nuclei of Caenorhabditis elegans. Journal of Radiation Research 44: 271-276
  • Sasagawa, Y., Urano, T., Kohara, Y., Takahashi, H., Higashitani, A. Caenorhabditis elegans RBX1 is essential for meiosis, mitotic chromosomal condensation and segregation, and cytokinesis. Genes to Cells 8: 857-872
  • Takanami, T., Mori, A., Takahashi, H., Horiuchi, S., Higashitani, A. Caenorhabditis elegans Ce-rdh-1/rad-51 functions after double-strand break formation of meiotic recombination. Chromosome Research 11: 125-135
  • Kitaya, Y., Kawai, M., Tsuruyama, J., Takahashi, H., Tani, A., Goto, E., Saito, T., Kiyota, M. The effect of gravity on surface temperatures of plant leaves. Plant, Cell and Environment 26: 497-503

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    2002年

  • Takahashi, N., Goto, N., Okada, K., Takahashi, H. Hydrotropism in abscisic acid, wavy, and gravitropic mutants of Arabidopsis thaliana. Planta 216: 203-211
  • Mizuno, H., Kobayashi, A., Fujii, N., Yamashita, M., Takahashi, H. Hydrotropic response and expression pattern of auxin-inducible gene, CS-IAA1, in the primary roots of clinorotated cucumber seedlings. Plant and Cell Physiology 43: 793-801
  • Miyamoto, N., Ookawa, T., Takahashi, H., Hirasawa, T. Water uptake and hydraulic properties of elongating cells in hydrotropically bending roots of Pisum sativum L. Plant and Cell Physiology 43: 393-401
  • Nishizawa, T., Chen, L., Higashitani, A,. Takahashi, H., Takeda, K., Suge, H. Responses of the first internodes of Hong Mang Mai wheat to ethylene, gibberellins and potassium. Plant Production Science 5: 93-100

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    2001年

  • Yamasaki, S., Fujii, N., Matsuura, S., Takahashi, H. The M locus and ethylene controlled sex determination in andromonoecious cucumber plants. Plant and Cell Physiology 42: 608-619
  • Chen, L., Nishizawa, T., Higashitani, A., Suge, H., Wakui, Y., Takeda, K., Takahashi H. A variety of wheat tolerant to deep-seeding conditions: Elongation of the first internode depends on the response to gibberellin and potassium. Plant, Cell and Environment 24: 469-476
  • Kitaya, Y., Kawai, M., Tsuruyama, J., Takahashi, H., Tani, A., Goto, E., Saito, T., Kiyota, M. The effect of gravity on surface temperature and net photosynthetic rate of plant leaves. Advances in Space Research 28: 659-664
  • Tani, A., Okuma, T., Goto, E., Kitaya, Y., Saito, T., Takahashi, H. Ground performance of air conditioning and water recycle system for a space plant box. Advances in Space Research 27: 1557-1562

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