研究業績
2019年
Tanaka-Takada N., Kobayashi A., Takahashi H., Kamiya T., Kinoshita T., Maeshima M. Plasma membrane-associated Ca2+-binding protein PCaP1 is involved in root hydrotropism of Arabidopsis thaliana. Plant and Cell Physiology doi:10.1093/pcp/pcz042.
Top Page
2018年
Kobayashi A., Kim H-J., Tomita Y., Miyazawa Y., Fujii N., Yano S., Yamazaki C., Kamada M., Kasahara H., Miyabayashi S., Shimazu T., Fusejima Y., Takahashi H. Circumnutational movement in rice coleoptiles involves the gravitropic response: analysis of an agravitropic mutant and space-grown seedlings. Physiologia Plantarum doi: 10.1111/ppl.12824.
Kojima S., Iwamoto M., Oiki S., Tochigi S., Takahashi H. Thylakoid membranes contain a non-selective channel permeable to small organic molecules. Journal of Biological Chemistry 293(20):7777-7785. doi: 10.1074/jbc.RA118.002367
Fujii N., Miyabayashi S., Sugita T., Kobayashi A., Yamazaki C., Miyazawa Y., Kamada M., Kasahara H., Osada I., Shimazu T., Fusejima Y., Higashibata A., Yamazaki T., Ishioka N., Takahashi H. Root-tip-mediated inhibition of hydrotropism is accompanied with the suppression of asymmetric expression of auxin-inducible genes in response to moisture gradients in cucumber roots. PLoS One 13(1):e0189827 DOI: 10.1371/journal.pone.0189827. eCollection 2018.
Top Page
2017年
Kowata H., Tochigi S.,Takahashi H. and Kojima S. Outer membrane permeability of cyanobacterium Synechocystis sp. PCC 6803: studies of passive diffusion of small organic nutrients reveal the absence of classical porins and intrinsically low permeability. Journal of Bacteriology DOI: 10.1128/JB.00371-17
Morohashi K., Okamoto M., Yamazaki C., Fujii N., Miyazawa Y., Kamada M., Kasahara H., Osada I., Shimazu T., Fusejima Y., Higashibata A., Yamazaki T., Ishioka N., Kobayashi A., and Takahashi H. Gravitropism interferes with hydrotropism via counteracting auxin dynamics in cucumber roots: Clinorotation and spaceflight experiments. New Phytologist 215(4):1476-1489. doi: 10.1111/nph.14689
Kohzuma K., Chiba M., Nagano S., Anai T., Ueda M.U., Oguchi R., Shirai K., Hanada K., Hikosaka K., Fujii N. Mutant selection in the self-incompatible plant radish (Raphanus sativus L. var. sativus) using two-step TILLING. Breeding Science 67(3):268-276. doi: http://doi.org/10.1270/jsbbs.16200
Nakajima Y.*, Nara Y.*, Kobayashi A.*, Sugita T., Miyazawa Y., Fujii N. and Takahashi H. Auxin transport and response requirements for root hydrotropism differ between plant species. Jornal of Experimental Botany 68(13):3441-3456. doi: 10.1093/jxb/erx193. *equal contribution
Dietrich D.*, Pang L.*, Kobayashi A.*, Fozard J.A., Boudolf V., Bhosale R., Antoni R., Nguyen T., Hiratsuka S., Fujii N., Miyazawa Y., Bae T-W., Wells D.M., Owen M.R., Band L.R., Dyson R.J., Jensen O.E., King J.R., Tracy S.R., Sturrock C.J., Mooney S.J., Roberts J.A., Bhalerao R.P., Dinneny J.R., Rodriguez P.L., Nagatani A., Hosokawa Y., Baskin T.I., Pridmore T.P., Veylder L.D., Takahashi H. and Bennett M.J. Root hydrotropism is controlled via a cortex-specific growth mechanism. Nature Plants 3: 17057 DOI: 10.1038/nplants.2017.57 *equal contribution
Top Page
2016年
Sugawara, E., Kojima, S., Nikaido, H. Klebsiella pneumoniae major porins OmpK35 and OmpK36 allow more efficient diffusion of β-lactams than their Escherichia coli homologs OmpF and OmpC. Journal of Bacteriology 198(23):3200-3208
Yamazaki, C., Fujii, N., Miyazawa, Y., Kamada, M., Kasahara, H., Osada, I., Shimazu, T., Fusejima, Y., Higashibata, A., Yamazaki, T., Ishioka, N., Takahashi, H. The gravity-induced re-localization of auxin efflux carrier CsPIN1 in cucumber seedlings: spaceflight experiments for immunohistochemical microscopy. npj Microgravity 2 Article number: 16030
Kojima, S., Muramoto, K., Kusano,T. Outer Membrane Proteins Derived from Non-cyanobacterial Lineage Cover the Peptidoglycan of Cyanophora paradoxa Cyanelles and Serve as a Cyanelle Diffusion Channel. The journal of Biological Chemistry 291(38):20198-20209.
Kojima, S., Hayashi, K., Tochigi, S., Kusano, T., Kaneko, J., Kamio, Y. Peptidoglycan-associated outer membrane protein Mep45 of rumen anaerobe Selenomonas ruminantium forms a non-specific diffusion pore via its C-terminal transmembrane domain. Bioscience, Biotechnology, and Biochemistry 80:1954-1959.
Kowata, H., Tochigi, S., Kusano, T., Kojima, S. Quantitative measurement of the outer membrane permeability in Escherichia coli lpp and tol-pal mutants defines the significance of Tol-Pal function for maintaining drug resistance. The journal of Antibiotics Advance online publication 11 May 2016
G.H.M., Sagor., Berberich, T., Kojima, S., Niitsu, M., Kusano, T. Spermine modulates the expression of two probable polyamine transporter genes and determines growth responses to cadaverine in Arabidopsis. Plant Cell Reports 35:1247-1257.
G.H.M., Sagor., Zhang, S., Kojima, S., Simm, S., Berberich, T., Kusano, T. Reducing cytoplasmic polyamine oxidase activity in Arabidopsis increases salt and drought tolerance by reducing reactive oxygen species production and increasing defense gene expression. Frontiers in Plant Science 7:214.
G.H.M., Sagor., Berberich, T., Tanaka, S., Nishiyama, M., Kanayama, Y., Kojima, S., Muramoto, K., Kusano, T. A novel strategy to produce sweeter tomato fruits with high sugar contents by fruit-specific expression of a single bZIP transcription factor gene. Plant Biotechnology Journal 14:1116-1126.
児島征司 グラム陰性細菌の多剤耐性― β-lactam 系抗生物質の外膜透過・排出速度の測定結果を例に理解する― 化学と生物 54(2):80-82.
Kim, H.J. Kobayashi, A., Fujii, N., Miyazawa, Y., Takahashi, H. Gravitropic response and circumnutation in pea (Pisum sativum) seedling roots. Physiologia Plantarum 157: 108–118.
Top Page
2014年
Moriwaki, T., Miyazawa, Y., Fujii, N., Takahashi, H. GNOM regulates root hydrotropism and phototropism independently of PIN-mediated auxin transport. Plant Science 215-216: 141-149.
Top Page
2013年
Yamazaki, C., Fujii N., Takahashi, H. Polar Auxin Transport: The Role of Auxin Transport and Distribution in Plant Gravimorphogenesis." Springer Berlin Heidelberg 25-44.
Iwata, S., Miyazawa, Y., Fujii N., Takahashi, H. MIZ1-regulated hydrotropism functions in the growth and survival of Arabidopsis thaliana under natural conditions. Annals of Botany 112 (1): 103-114
Moriwaki, T., Miyazawa, Y., Kobayashi, A., Takahashi, H. Molecular mechanisms of hydrotropism in seedling roots of Arabidopsis thaliana (Brassicaceae). American Journal of Botany 100.1: 25-34
Top Page
2012年
Miyazawa, Y., Moriwaki, T., Uchida, M., Kobayashi, A.,
Fujii N., Takahashi, H. Overexprresion of MIZU-KUSSEI1 enhances root hydrotropic response by retaining cell viability under hydrostimulated condition in Arabidopsis thaliana. Plant and Cell Physiology 53(11): 1926-1933
Nakayama, M., Kaneko, Y., Miyazawa, Y., Fujii, N., Higashitani, N., Wada, S., Ishida, H., Yoshimoto, K., Shirasu, K., Yamada, K., Nishimura, M., Takahashi, H. A possible involvement of autophagy in amyloplast degradation in columella cells during hydrotropic response of Arabidopsis roots. Planta 236: 999-1012
Moriwaki, T., Miyazawa, Y.,
Fujii N., Takahashi, H. Light and abscisic acid signaling are
integrated by MIZ1 gene expression and regulate hydrotropic response in
roots of Arabidopsis thaliana. Plant Cell and Environment 20: 79-83.
Yamazaki, T., Miyazawa, Y.,
Kobayashi, A., Moriwaki, T., Fujii, N., Takahashi, H. MIZ1, an
essential protein for root hydrotropism, is associated with the cytoplasmic
face of the endoplasmic reticulum membrane in Arabidopsis thaliana.
FEBS Letters 586: 398-402
Watanabe,
C., Fujii, N., Yanai, K., Hotta, T., Kim, D-H., Kamada, M., Sasagawa-Saito, Y.,
Nishimura, T., Koshiba, T., Miyazawa, Y., Kim, K-M., Takahashi, H.
Gravistimulation changes the accumulation pattern of the CsPIN1 auxin efflux
facilitator in the endodermis of the transition zone in cucumber
seedlings. Plant Physiology 158: 239-251
Iwata,
S., Miyazawa, Y., Takahashi, H. MIZU-KUSSEI1 plays an essential role in
the hydrotropism of lateral roots in Arabidopsis thaliana. Environmental
and Experimental Botany 75: 167-172
山崎聖司、真鍋和人、藤井伸治、高橋秀幸. ウリ科植物の花の性分化のエチレン制御と遺伝モデル. 植物の生長調節 47(1) : 24-33
Top Page
2011年
Moriwaki, T., Miyazawa, Y.,
Kobayashi, A., Uchida, M., Watanabe, C., Fujii, N., Takahashi, T. Hormonal
regulation of lateral root development in Arabidopsis modulated by MIZ1
and requirement of GNOM activity for MIZ1 function. Plant
Physiology 157: 1209-1220
Miyazawa, Y., Yamazaki, T.,
Moriwaki, T., Takahashi, H. Root tropisms: its mechanism and possible
functions in drought avoidance. In Turkan I. (eds.) Plant responses to
drought and salinity stress: development in a post-genomic era, Advances in
Botanical Research 57: 349-375
Kato,
F., Araki, M., Miyazawa, Y., Fujii, N., Takeda, K., Suge, H., Takahashi, H.
Factors responsible for deep-sowing tolerance in wheat
seedlings: Varietal differences in cell proliferation and the coordinated
synchronization of epidermal cell expansion and cortical cell division for the
gibberellin-mediated elongation of first internodes. Annals of Botany 108:
439-447
Top Page
2010年
Sasagawa-Saito,
Y., Kitazawa, D., Miyazawa, Y., Fujii, N., Takahashi, H. Differential
accumulation of CsARF5 protein during peg formation in cucumber seedlings. Biological
Sciences in Space 24: 153-162
Sakata,
T., Oshino, T., Miura, S., Tomabechi, M., Tsunaga, Y., Higashitani, N.,
Miyazawa, Y., Takahashi, H., Watanabe, M., Higashitani, A. Auxins
reverse plant male sterility caused by high temperatures. Proceedings of the
National Academy of Sciences of the U.S.A. 107: 8569-8574
Moriwaki,
T., Miyazawa, Y., Takahashi, H. Transcriptome analysis of gene
expression during the hydrotropic response in Arabidopsis seedlings. Environmental
and Experimental Botany 69: 148-157
Nishio, S., Moriguchi, R.,
Ikeda, H., Takahashi, H., Takahashi, H., Fujii, N., Guilfoyle, T.J., Kanahama,
K., Kanayama, Y. Expression analysis of the auxin efflux carrier family
in tomato fruit development. Planta 232: 755-764
Top Page
2009年
Miyazawa,
Y., Takahashi, A., Kobayashi, A., Kaneyasu, T., Fujii, N., Takahashi, H.
GNOM-mediated vesicular trafficking plays an essential role in hydrotropism of
Arabidopsis roots. Plant Physiology 149: 835-840
Miyazawa,
Y., Ito, Y., Moriwaki, T., Kobayashi, A., Fujii, N., Takahashi, H. A
molecular mechanism unique to hydrotropism in roots. Plant Science 177:
297-301
Takahashi,
H., Miyazawa, Y., Fujii, N. Hormonal interactions during root tropic
growth: hydrotropism versus gravitropism. Plant Molecular Biology 69:
489-502
Top Page
2008年
Miyazawa,
Y., Sakashita, T., Funayama, T., Hamada, N., Negishi, H., Kobayashi, A.,
Kaneyasu, T., Ooba, A., Morohashi, K., Kakizaki, T., Wada, S., Kobayashi, Y.,
Fujii, N., Takahashi, H. Effects of locally targeted heavy-ion and laser
microbeam on root hydrotropism in Arabidopsis thaliana. Journal of
Radiation Research 49: 373-379
Kitazawa,
D., Miyazawa, Y., Fujii, N., Hoshino, A., Iida, S., Nitasaka, E., Takahashi, H.
The gravity-regulated growth of axillary bud is mediated by a mechanism
different from decapitation-induced release. Plant and Cell Physiology
49: 891-900
Shimizu, M., Miyazawa, Y., Fujii, N., Takahashi, H.
p-Chlorophenoxy- isobutyric acid impairs auxin response for
gravity-regulated peg formation in cucumber seedlings. Journal of Plant
Research 121: 107-114
Kitazawa, D., Miyazawa, Y., Fujii, N., Nitasaka, E.,
Takahashi, H. Characterization of a novel gravitropic mutant of morning
glory, weeping2. Advances in Space Research 42: 1050-1059
Top Page
2007年
Kobayashi,
A., Takahashi, A., Kakimoto, Y., Miyazawa, Y., Fujii, N., Higashitani, A.,
Takahashi, H. A gene essential for hydrotropism in roots. Proceedings
of the National Academy of Sciences of the U.S.A. 104: 4724-4729
Saito,
S., Fujii, N., Miyazawa, Y., Yamasaki, S., Matsuura, S., Mizusawa, H., Fujita,
Y., Takahashi, H. Correlation between development of female flower buds
and expression of the CS-ACS2 gene in cucumber plants. Journal of Experimental
Botany 58: 2897-2907
Kaneyasu,
T., Kobayashi, A., Nakayama, M., Fujii, N., Takahashi, H., Miyazawa, Y.
Auxin response, but not its polar transport, plays a role in hydrotropim of Arabidopsis
thaliana. Journal of Experimental Botany 58: 1143-1150
Miyazawa,
Y., Takahashi, H. How do Arabidopsis roots differentiate hydrotropism
from gravitropism? Plant Signaling and Behavior 2: 388-389
Fujii, N., Saito, Y., Miyazawa, Y., Takahashi, H.
Light inhibits gravity-regulated peg formation and asymmetric mRNA accumulation
of auxin-inducible CsIAA1 in the cortex of the transition zone in
cucumber seedlings. Advances in Space Research 39: 1147-1153
Shimizu, M., Suzuki, K., Miyazawa, Y., Fujii, N.,
Takahashi, H. Differential accumulation of the mRNA of the
auxin-repressed gene CsGRP1 and the auxin-induced peg formation during
gravimorphogenesis of cucumber seedlings. Planta 225: 13-22
Top Page
2006年
Kitaya, Y., Kawai, M., Takahashi, H., Tani, A., Goto, E.,
Saito, T., Shibuya, T., Kiyota, M. Heat and gas exchanges between plants
and atmosphere under microgravity. Annals of New York Academy of Sciences
1077: 244-255
Masuko, H., Endo, M., Saito, H., Hakozaki, H., Park, J-I.,
Kawagishi-Kobayashi, M., Takada, Y., Okabe, T., Kamada, M., Takahashi, H.,
Higashitani, A., Watanabe, M. Anther-specific genes, which expressed
through microsporogenesis, are temporally and spatially regulated in model
legume, Lotus japonicus. Genes and Genetic Systems 81: 57-62
Top Page
2005年
Kitazawa, D., Hatakeda, Y., Kamada, M., Fujii, N.,
Miyazawa, Y., Hoshino, A., Iida, S., Fukaki, H., Morita, M.T., Tasaka, M., Suge,
H., Takahashi, H. Shoot circumnutation and winding movements require
gravisensing cells. Proceedings of the National Academy of Sciences of the
U.S.A.102: 18742-18747
Abiko, M., Akibayashi, K., Sakata, T., Kimura, M.,
Kihata, M., Itoh, K., Asamizu, E., Sato, S., Takahashi, H., Higashitani, A.
High-temperature induction of male sterility during barley (Hordeum
vulgare L.) anther development is mediated by transcriptional inhibition. Sexual
Plant Reproduction 18: 91-100
Sasagawa, Y., Saito, Y., Shimizu, M., Takahashi, H.,
Higashitani, A. Perturbation of oocyte meiotic division by hypergravity
in Caenorhabditis elegans. Biological Sciences in Space 19:
155-162
高橋秀幸、藤井伸治、宮沢豊
「微小重力下における根の水分屈性とオーキシン動態」 生物工学 83:
560-564
Saito, Y., Yamasaki, S., Fujii, N., Takahashi, H. Possible
involvement of CS-ACS1 and ethylene in auxin-induced peg formation of
cucumber seedlings. Annals of Botany 95: 413-422
Yamasaki, S., Fujii, N., Takahashi, H. Hormonal regulation of
sex expression in plants. Vitamins and Hormones vol. 72: 79-110
Top Page
2004年
Saito, Y., Yamasaki, S., Fujii, N., Hagen, G., Guilfoyle, T.,
Takahashi, H. Isolation of cucumber CsARF cDNAs and expression of
the corresponding mRNAs during gravity-regulated morphogenesis of cucumber
seedlings. Journal of Experimental Botany 55: 1315-1323
Hakozaki, H., Endo, M.,
Masuko, H., Park, J-I., Ito, H., Uchida, M., Kamada, M., Takahashi, H.,
Higashitani, A., Watanabe, M. Cloning and expression pattern of a novel
microspore-specific gene encoding hypersensitive-induced response protein
(LjHIR1) from the model legume, Lotus japonicus. Genes and Genetic
Systems 79: 307-310
Endo, M., Tsuchiya, T.,
Saito, H., Matsubara, H., Hakozaki, H., Masuko, H., Kamada, M., Higashitani, A.,
Takahashi, H., Fukuda, H., Demura, T., Watanabe, M. Identification and
molecular characterization of novel anther-specific genes in Oryza sativa
L. by using cDNA microarray. Genes and Genetic Systems 79: 213-226
Top Page
2003年
Kamada, M., Yamasaki, S., Fujii, N., Higashitani, A., Takahashi, H.
Gravity-induced modification of auxin transport and distribution for peg
formation in cucumber seedlings: possible roles for CS-AUX1 and CS-PIN1. Planta
218: 15-26
Takahashi, N., Yamazaki, Y., Kobayashi, A., Higashitani, A.,
Takahashi, H. Hydrotropism interacts with gravitropism by degrading
amyloplasts in seedling roots of Arabidopsis and radish. Plant Physiology
132: 805-810
Yamasaki, S., Fujii, N., Takahashi, H. Photoperiodic
regulation of CS-ACS2, CS-ACS4, and CS-ERS gene expression
contributions to the femaleness of cucumber flowers through diurnal ethylene
production under short-day conditions. Plant Cell and Environment
26: 537-546.
Yamasaki, S., Fujii, N., Takahashi, H. Characterization of
ethylene effects on sex determination in cucumber plants. Sexual Plant
Reproduction 16: 103-111.
Hatakeda, Y., Kamada, M., Goto, N., Fukaki, H., Tasaka, M., Suge, H.
Takahashi, H. Gravitropic response plays an important role in the
nutational movements of the shoots of Pharbitis nil and Arabidopsis
thaliana. Physiologia Plantarum 118: 464-473
Chen, L., Higashitani, A., Suge, H., Takeda, K., Takahashi, H.
Spiral growth and cell wall properties of the gibberellin-treated first
internodes in the seedlings of a wheat cultivar tolerant to deep-sowing
conditions. Physiologia Plantarum 118: 147-155
Tsuda, S., Miyamoto, N.,
Takahashi, H., Ishihara, K., Hirasawa, T. Roots of Pisum sativum
L. exhibit hydrotropism in response to a water potential gradient in
vermiculite. Annals of Botany 92: 767-770
Takanami, T., Zhang, Y.,
Aoki, H., Abe, T., Yoshida, S., Takahashi, H., Horiuchi, S., Higashitani, A.
Efficient repair of DNA damage induced by heavy ion particles in meiotic
prophase I nuclei of Caenorhabditis elegans. Journal of Radiation
Research 44: 271-276
Sasagawa, Y., Urano, T.,
Kohara, Y., Takahashi, H., Higashitani, A. Caenorhabditis elegans
RBX1 is essential for meiosis, mitotic chromosomal condensation and
segregation, and cytokinesis. Genes to Cells 8: 857-872
Takanami, T., Mori, A.,
Takahashi, H., Horiuchi, S., Higashitani, A. Caenorhabditis elegans
Ce-rdh-1/rad-51 functions after double-strand break formation of meiotic
recombination. Chromosome Research 11: 125-135
Kitaya, Y., Kawai, M.,
Tsuruyama, J., Takahashi, H., Tani, A., Goto, E., Saito, T., Kiyota, M. The
effect of gravity on surface temperatures of plant leaves. Plant, Cell and
Environment 26: 497-503
Top Page
2002年
Takahashi, N., Goto, N.,
Okada, K., Takahashi, H. Hydrotropism in abscisic acid, wavy, and
gravitropic mutants of Arabidopsis thaliana. Planta 216: 203-211
Mizuno, H., Kobayashi, A.,
Fujii, N., Yamashita, M., Takahashi, H. Hydrotropic response and
expression pattern of auxin-inducible gene, CS-IAA1, in the primary
roots of clinorotated cucumber seedlings. Plant and Cell Physiology 43:
793-801
Miyamoto, N., Ookawa, T.,
Takahashi, H., Hirasawa, T. Water uptake and hydraulic properties of
elongating cells in hydrotropically bending roots of Pisum sativum L. Plant
and Cell Physiology 43: 393-401
Nishizawa, T., Chen, L.,
Higashitani, A,. Takahashi, H., Takeda, K., Suge, H. Responses of the
first internodes of Hong Mang Mai wheat to ethylene, gibberellins and potassium. Plant
Production Science 5: 93-100
Top Page
2001年
Yamasaki, S., Fujii, N.,
Matsuura, S., Takahashi, H. The M locus and ethylene controlled
sex determination in andromonoecious cucumber plants. Plant and Cell
Physiology 42: 608-619
Chen, L., Nishizawa, T.,
Higashitani, A., Suge, H., Wakui, Y., Takeda, K., Takahashi H. A variety
of wheat tolerant to deep-seeding conditions: Elongation of the first internode
depends on the response to gibberellin and potassium. Plant, Cell and
Environment 24: 469-476
Kitaya, Y., Kawai, M.,
Tsuruyama, J., Takahashi, H., Tani, A., Goto, E., Saito, T., Kiyota, M.
The effect of gravity on surface temperature and net photosynthetic rate of
plant leaves. Advances in Space Research 28: 659-664
Tani, A., Okuma, T., Goto,
E., Kitaya, Y., Saito, T., Takahashi, H. Ground performance of air
conditioning and water recycle system for a space plant box. Advances in
Space Research 27: 1557-1562
Top Page